The pre-mRNA to splice
Splicing options. Set skipSpliceSiteValidation to true to skip splice site validation. Useful for mutation analysis where splice sites may be intentionally disrupted or mutations are far from splice boundaries.
Optional
skip
Splices a pre-mRNA by removing introns and joining exons to produce mature mRNA. This function performs the core splicing operation that removes introns and joins exons while maintaining proper reading frame.